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KMID : 0613820110210121716
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Journal of Life Science
2011 Volume.21 No. 12 p.1716 ~ p.1720
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Isolation of Bacillus subtilis CK-2 Hydrolysing Various Organic Materials
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Kim Chul-Ho
Lee Sang-Hyup
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Abstract
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A bacterium hydrolysing various organic materials including cellulose, protein, starch and lipid was isolated. The isolate was identified as Bacillus subtilis, and named Bacillus subtilis CK-2 in this paper. This bacterium showed optimal growth at 40~45℃pH 6~9, and 0~3% of NaCl. B. subtilis CK-2 seemed to synthesis highly active autolysin. The hydrolytic enzymes produced by B. subtilis CK-2 were primary enzymes because extracellular enzyme activities varied similarly to the growth curve. The hydrolytic enzymes seemed to be stable at basic pH conditions. From these results, B. subtilis CK-2 was found to bea useful bacterial agent for composting, or for use in feed-production waste in agriculture, fishery, forest materials, livestock farming, and food.
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KEYWORD
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Bacillus subtilis, cellulase, protease, amylase, lipase
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